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  • : MAB4334
  • : 100 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

Species Reactivity Key Applications Host Format Antibody Type
H, Pm, not M, not R FC, IF, IP, IHC, IH(P), FUNC Mouse Purified Monoclonal Antibody
Description:
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
Epitope:
conformational extracellular epitope
Immunogen:
Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
Clone:
Isotype:
IgG2aκ
Background Information:
UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905).
Species Reactivity:
  • Human
  • Primate
Non-Reactive Species:
  • Mouse
  • Rat
Application Notes:
Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.

Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.

Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.

Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.

IHC on frozen and paraffin sections: 1 - 10 μg/mL.

Optimal working dilutions must be determined by end user.
Presentation:
The immunoglobulin was purified by protein A Sepharose™ chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain at 2-8°C for up 12 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • ABCB1
  • PGY1
  • MGC163296
  • CD243
  • GP170
  • P-gp
  • MDR1
  • CLCS
  • ABC20
  • EC 3.6.3.44
Alternate Names:
P-glycoprotein; CD243; p170; Pgp
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Affects Function
Entrez Gene Summary:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
UniProt Summary:
FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
Brand Family:
Chemicon
Product Name:
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
Applications:
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).

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