Ordering Information
Product Images
Immunohistochemistry Analysis:
Staining of anti-BMP-7, clone 2A10 on normal human kidney medulla (left) and normal human kidney cortex (right). T...
Western Blot Analysis:
Mouse kidney cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-BMP-7, clone 2A10 (1 µ...
Anti-BMP-7, clone 2A10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
Function Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Subunit structure Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1 By similarity.
Subcellular location Secreted.
Tissue specificity Expressed in the kidneys and bladder. Lower levels seen in the brain.
Post-translational modification Several N-termini starting a ... see more »
Subunit structure Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1 By similarity.
Subcellular location Secreted.
Tissue specificity Expressed in the kidneys and bladder. Lower levels seen in the brain.
Post-translational modification Several N-termini starting a ... see more »
Description:
Anti-BMP-7, clone 2A10
Background Information:
Bone morphogenetic protein-7 (BMP-7), also known as osteogenic protein-1, is a member of the TGF- superfamily, and is widely expressed during embryonic development. Like other members of the bone morphogenetic protein family, BMP-7 works synergistically with BMP-2 to play a key role in the differentiation of mesenchymal cells into bone and cartilage tissues by actively recruiting stem cells from the surrounding tissue, thereby initiating the bone formation cascade. In adult cells, BMP-7 is predominately expressed in the brain, kidneys, and bladder.
Clone:
2A10
Specificity:
This monoclonal antibody recognizes BMP-7.
Key Applications:
- Western Blotting
- Immunohistochemistry
Application Notes:
Immunohistochemistry: Recommended working dilution is 1:50.
Western Blotting: Recommended working concentration is 1 – 2 µg/mL.
Western Blotting: Recommended working concentration is 1 – 2 µg/mL.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Reacts with human and mouse. Reactivity with other species has not been tested.
Isotype:
IgG1κ
Immunogen:
Recombinant full-length BMP-7 protein.
Molecular Weight:
46 kDa
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4),
150 mM NaCl, and 0.05% sodium azide.
150 mM NaCl, and 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
Antibody Category:
Stem Cell Research
Antibody Sub-Category:
Mesenchymal Stem Cells
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Type:
Mesenchymal Stem Cells
Stem Cell Workflow Stage:
Characterization
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein G Purification