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Immunohistochemistry Analysis:
Staining of anti-BMP-7, clone 2A10 on normal human kidney medulla (left) and normal human kidney cortex (right). ...
Western Blot Analysis:
Mouse kidney cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-BMP-7, clone 2A10 (1 ...
Anti-BMP-7, clone 2A10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-BMP-7, clone 2A10
Specificity:
This monoclonal antibody recognizes BMP-7.
Molecular Weight:
46 kDa
Immunogen:
Recombinant full-length BMP-7 protein.
Clone:
2A10
Isotype:
IgG1κ
Background Information:
Bone morphogenetic protein-7 (BMP-7), also known as osteogenic protein-1, is a member of the TGF- superfamily, and is widely expressed during embryonic development. Like other members of the bone morphogenetic protein family, BMP-7 works synergistically with BMP-2 to play a key role in the differentiation of mesenchymal cells into bone and cartilage tissues by actively recruiting stem cells from the surrounding tissue, thereby initiating the bone formation cascade. In adult cells, BMP-7 is predominately expressed in the brain, kidneys, and bladder.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Reacts with human and mouse. Reactivity with other species has not been tested.
Application Notes:
Immunohistochemistry: Recommended working dilution is 1:50.
Western Blotting: Recommended working concentration is 1 – 2 µg/mL.
Western Blotting: Recommended working concentration is 1 – 2 µg/mL.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4),
150 mM NaCl, and 0.05% sodium azide.
150 mM NaCl, and 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
UniProt Number:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry
UniProt Summary:
Function Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Subunit structure Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1 By similarity.
Subcellular location Secreted.
Tissue specificity Expressed in the kidneys and bladder. Lower levels seen in the brain.
Post-translational modification Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (Ref.5).
Pharmaceutical use Available under the names Osigraft (Stryker). Its use is indicated in the treatment of tibial non-union of at least 9 month duration, secondary to trauma, in skeletally mature patients, in cases where autograft has failed or is unfeasible.
Sequence similarities Belongs to the TGF-beta family.
Subunit structure Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1 By similarity.
Subcellular location Secreted.
Tissue specificity Expressed in the kidneys and bladder. Lower levels seen in the brain.
Post-translational modification Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (Ref.5).
Pharmaceutical use Available under the names Osigraft (Stryker). Its use is indicated in the treatment of tibial non-union of at least 9 month duration, secondary to trauma, in skeletally mature patients, in cases where autograft has failed or is unfeasible.
Sequence similarities Belongs to the TGF-beta family.
Stem Cell Type:
Mesenchymal Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Product Name:
Anti-BMP-7, clone 2A10
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody