Ordering Information
Anti-Caspase 3, large subunit & proform, clone 4-1-18
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
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Entrez Gene Number:
Entrez Gene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 an
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Description:
Anti-Caspase 3, large subunit & proform, clone 4-1-18
Alternate Names:
CPP32
Clone:
4-1-18
Specificity:
Recognizes the full length 32 kDa caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Caspase-3 is one of the most extensively studied enzyme in apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
Western blot: 1:1000. The antibody will detect a band at 32 kDa corresponding to the inactive unprocessed form of caspase 3 as well as a 17-22 kDa band corresponding to the large subunit of active caspase 3.
Immunohistochemistry (frozen and paraffin): 1:500.
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and paraffin): 1:500.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Human
Isotype:
IgG2aκ
Immunogen:
Recombinant full-length human caspase-3 protein
Epitope:
large subunit & proform
Control:
POSITIVE CONTROL: 293 (human embryonic kidney) cell lysates
Presentation:
Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Gene Symbol:
- CASP3
- apopain
- CASP-3
- procaspase3
- CPP32B
- SCA-1
- CPP-32
- Apopain
- CPP32
- Yama
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17
- Caspase-3 subunit p12].
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- Caspases
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/ml
Antibody Type:
Monoclonal Antibody