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Mouse anti-Synaptophysin (Catalogue No. MAB5258) localization of synaptophysin in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University o...
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-synaptophysin (1:100...
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Anti-Synaptophysin, clone SY38
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, B, F, H, M, R | FC, IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Synaptophysin, clone SY38
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
Molecular Weight:
38 kDa
Immunogen:
Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
Clone:
SY38
Isotype:
IgG1κ
Background Information:
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
Species Reactivity:
- Avian
- Bovine
- Fish
- Human
- Mouse
- Rat
Species Reactivity Note:
Avian, Bovine, Human, Mouse,Rat, and Fish
Application Notes:
Flow Cytometry (FACS):
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
Control:
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Quality Assurance:
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
SYP
Alternate Names:
- synaptophysin
- Synaptophysin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
Brand Family:
Chemicon
Product Name:
Anti-Synaptophysin, clone SY38
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
20 µg
Format:
Purified
Host:
Mouse