画像情報
Mouse anti-Synaptophysin (Catalogue No. MAB5258) localization of synaptophysin in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University o...
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-synaptophysin (1:100...
)
)
)
Anti-Synaptophysin, clone SY38)
当該製品は右記製品の推奨代替品です: CBL203| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M, R, B, Av, F | WB, IC, IH | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, i ... もっと見る »
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, i ... もっと見る »
Entrez Gene Number:
Entrez Gene Summary
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
Description:
Anti-Synaptophysin, clone SY38)
Replaces:
CBL203
Background Information:
Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. The localization and ubiquitous expression of this protein in neuronal tissue makes it an excellent marker for neurons and neuroendocrine tumors. The gene for this protein is located on the X chromosome (Xp11.23-p11.22). The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.
Alternate Names:
Major synaptic vesicle protein p38
Clone:
clone SY38)
Specificity:
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
Application Notes:
Immunohistochemistry: For relatively cytoplasm rich neuroendocrinic tumors a final concentration of 1 μg/mL is recommended. A final concentration of 2 μg/mL is recommended for cytoplasm deficient tumors.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
- Avian
- Fish
Isotype:
IgG1κ
Immunogen:
Vesicular fraction of bovine brain
Molecular Weight:
38 kDa
Control:
Positive Control: Pancreas
Negative Control: Normal mouse serum
Negative Control: Normal mouse serum
Presentation:
Purified mouse monoclonal IgG1κ liquid in buffer containing 20 mM sodium phosphate buffer, 5 mg/mL BSA, with 0.01% methylisothiazolone MIT (w/v), pH 7.5.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- SYP
- synaptophysin
- Synaptophysin
Quality Assurance:
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this antibody detected synaptophysin on 10 ug of mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this antibody detected synaptophysin on 10 ug of mouse brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Synapse & Synaptic Biology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody