Ordering Information
- : MABS107
- : 100 µL
Product Images
Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of A431 and HeLa cells using Anti-Ubiquitin K11 linkage, clone...
Western Blotting Analysis:
Representative lot data.
K11-only Ubn-ubiquitinylated HM substrate protein synthesized using K11-only mutant ub...
. Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains both the cytosol and nucleus (with intense staining on nucleus).'))
, K48 Ub 2-7 (Enzo BioMol UW8860, lane 2), and K63 Ub 2-7 (Enzo BioMol UW9570, lane 3) lysates were probed with Anti-Ubiquitin K11 linkage, clone 2A3/2E6 and Anti-Ubiquitin, Lys48-Specific, clone Apu2 (05-1307) (1:1,000 dilution). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system. <br />Arrow indicates ubiquitinylated proteins. Anti-Ubiquitin K11 linkage, clone 2A3/2E6 was used for the western blot on the left panel while Anti-Ubiquitin, Lys48-Specific, clone Apu2 was used for the western blot on right panel as the control.'))
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Anti-Ubiquitin K11 linkage, clone 2A3/2E6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, A | WB, IP, ICC | Rabbit | Purified | Monoclonal Antibody |
Description:
Anti-Ubiquitin K11 linkage Antibody, clone 2A3/2E6 | MABS107
Promotional Text:
Molecular Weight:
various
Immunogen:
Full length di-ubiquitin dimer linked at K11
Clone:
2A3/2E6
Isotype:
IgG
Background Information:
Recently, the lysine11 (K11) linkage of ubiquitin has been shown to be specifically targeted by the Anaphase-Promoting Complex (APC) E3 ubiquitin ligase for catalyzed ubiquitination for mitosis. Antibodies specific to the K11-linkage have shown that the formation of the K11 chains are greatly increased in mitotic human cells in the presence of APC substrate degradation. K11-linked ubiquitin chains seem to have essential regulatory control over mitotic protein degradation.
Species Reactivity:
- Human
- All
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with all species based on 100% sequence homology.
Application Notes:
Immunoprecipitation Analysis: A representative lot was used to detect Ubiquitin K11 linkage in MuRF1.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ubiquitin K11 linkage in HeLa and A431 cells.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ubiquitin K11 linkage in HeLa and A431 cells.
Control:
K11-only Ubn-ubiquitinylated HM substrate protein
Quality Assurance:
Evaluated by Western Blot in K11-only Ubn-ubiquitinylated HM substrate protein.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Ubiquitin K11 linkage on 10 µg of K11-only Ubn-ubiquitinylated HM substrate protein.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Ubiquitin K11 linkage on 10 µg of K11-only Ubn-ubiquitinylated HM substrate protein.
Purification Method:
Protein A
Presentation:
Purified recombinant rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Gene Symbol:
Lys11
Alternate Names:
- Ubiquitin K11 linkage
- Lysine 11
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
- Immunocytochemistry
Product Name:
Anti-Ubiquitin K11 linkage, clone 2A3/2E6
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit