Ordering Information
- : MBN-41K-1OPG
- : 96-well plate
MILLIPLEX MAP Mouse OPG - Single Plex - Metabolism Assay
Description:
MILLIPLEX MAP Mouse OPG - Single Plex
Trade Name:
MILLIPLEX
Qty/Pk:
96-well plate
Background Information:
This single plex assay kit is to be used for the quantitative measurement of mouse OPG in cell/tissue culture supernatant and mouse serum/plasma samples. Osteoprotegerin (OPG, also known as OCIF or osteocalstogenesis inhibitory factor) is a soluble member of t
Applications:
This MILLIPLEX® MAP Mouse OPG - Single Plex is a Metabolism single plex assay. The analyte available for this kit is Osteoprotegerin.
Key Applications:
Multiplexing
Application Notes:
25 µL or less sample volume, Overnight assay
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
UniProt Summary:
FUNCTION: SwissProt: O00300 # Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
SIZE: 401 amino acids; 46040 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
PTM: N-glycosylated. Contains sialic acid residues. & The N-terminus is blocked.
DISEASE: "SwissProt: O00300 # Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also called hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal."
SIMILARITY: SwissProt: O00300 ## Contains 2 death domains. & Contains 4 TNFR-Cys repeats.
SIZE: 401 amino acids; 46040 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
PTM: N-glycosylated. Contains sialic acid residues. & The N-terminus is blocked.
DISEASE: "SwissProt: O00300 # Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also called hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal."
SIMILARITY: SwissProt: O00300 ## Contains 2 death domains. & Contains 4 TNFR-Cys repeats.
Linearity of Dilution:
1.12
Sensitivity:
2.3 pg/mL
Cross Reactivity:
Mouse OPG assay shows reactivity with recombinant rat OPG.
Product Name:
MILLIPLEX MAP Mouse OPG - Single Plex
Standard Curve Range:
10–40,000 pg/mL
Analytes Available:
Osteoprotegerin
Configuration:
Single Plex
UniProt Number:
Panel Type:
Non-Magnetic Bone
Precision, %:
- Inter-assay: 6.0
- Intra-assay: 3.1
Accuracy:
0.83
Detection Methods:
- Fluorescent
- Luminex xMAP
Assay Panel:
Everything you need in a single kit.