Ordering Information
- : MBN-41K-1RANKL
- : 96-well plate
MILLIPLEX MAP Mouse RANKL - Single Plex - Metabolism Assay
Description:
MILLIPLEX MAP Mouse RANKL - Single Plex
Trade Name:
MILLIPLEX
Qty/Pk:
96-well plate
Background Information:
This single plex assay kit is to be used for the quantitative measurement of mouse RANKL in cell/tissue culture supernatant and mouse serum/plasma samples. RANKL (receptor activator of nuclear factor-kappab ligand, also known as TRANCE) is a member of the TNF
Applications:
This MILLIPLEX® MAP Mouse RANKL - Single Plex is a Metabolism single plex assay. The analyte available for this kit is RANKL.
Key Applications:
Multiplexing
Application Notes:
25 µL or less sample volume, Overnight assay
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
UniProt Summary:
FUNCTION: SwissProt: O14788 # Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
SIZE: 317 amino acids; 35478 Da
SUBUNIT: Homotrimer (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type II membrane protein. & Isoform 3: Cell membrane; Single-pass type II membrane protein. & Isoform 2: Secreted (By similarity). & Processed isoform 1: Secreted (By similarity).
TISSUE SPECIFICITY: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
DISEASE: "SwissProt: O14788 # Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non- functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development."
SIMILARITY: SwissProt: O14788 ## Belongs to the tumor necrosis factor family.
SIZE: 317 amino acids; 35478 Da
SUBUNIT: Homotrimer (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type II membrane protein. & Isoform 3: Cell membrane; Single-pass type II membrane protein. & Isoform 2: Secreted (By similarity). & Processed isoform 1: Secreted (By similarity).
TISSUE SPECIFICITY: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
DISEASE: "SwissProt: O14788 # Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non- functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development."
SIMILARITY: SwissProt: O14788 ## Belongs to the tumor necrosis factor family.
Linearity of Dilution:
0.932
Sensitivity:
3.0 pg/mL
Cross Reactivity:
Mouse RANKL assay shows reactivity with recombinant rat RANKL.
Product Name:
MILLIPLEX MAP Mouse RANKL - Single Plex
Standard Curve Range:
10–40,000 pg/mL
Analytes Available:
RANKL
Configuration:
Single Plex
UniProt Number:
Panel Type:
Non-Magnetic Bone
Precision, %:
- Inter-assay: 7.1
- Intra-assay: 3.1
Accuracy:
1.13
Detection Methods:
- Fluorescent
- Luminex xMAP
Assay Panel:
Everything you need in a single kit.