Muse EGFR-RTK Activation Dual Detection

Muse™ EGFR-RTK Activation Dual Detection

Muse

50 Tests

EMD Millipore’s Muse™ Activation Dual Detection kits are a series of products which include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Two parameter analysis allows specific detection of phosphorylation and eliminates false positives while enhancing the signal to noise ratio. The Muse™ EGFR-RTK Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-EGFR (Tyr1173)-Alexa Fluor®555 and an anti-EGFR-PECy5 conjugated antibody to measure total levels of EGFR expression. This two color kit is designed to detect the extent of EGFR pathway activation by measuring EGFR phosphorylation relative to the total EGFR expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of EGFR activation after stimulation. Moreover, simultaneous measurement of both total and phospho-EGFR confirms target specificity of the phosphorylation event. All Muse™ Dual Detection kits are optimized, and designed for use, on the Muse™ Cell Analyzer. Both antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for EGFR signaling analysis. Data generated using the Muse™ Cell Analyzer with the Muse™ software provides:
• Percentage of inactivated cells
• Percentage of activated cells (via phosphorylation)
• Percentage of non-expressing cells

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Muse™ EGFR-RTK Activation Dual Detection

Assay Specific Module

• Bioprocess
• Research