Muse PI3K Activation Dual Detection Kit

Muse™ PI3K Activation Dual Detection Kit

Muse

50 Tests

EMD Millipore’s Muse™ Activation Dual Detection kits are a series of products which include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Two parameter analysis allows specific detection of phosphorylation and eliminates false positives while enhancing the signal to noise ratio. The Muse™ PI3K Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-Akt (Ser473), Alexa Fluor®555 and an anti-Akt, PECy5 conjugated antibody to measure total levels of Akt. This two color kit is designed to measure the extent of Akt phosphorylation relative to the total Akt expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PI3K activation after stimulation. Moreover, simultaneous measurement of both total and phospho-Akt confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population. All Muse™ Dual Detection kits are optimized, and designed for use, on the Muse™ Cell Analyzer. The antibody pair provided in the kit has been carefully titrated to ensure the ability to measure total and phospho-Akt simultaneously on the same protein for accurate determination of protein level and activation. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for Akt signaling analysis. Data generated using the Muse™ Cell Analyzer with the Muse™ software provides:
• Percentage of inactivated cells
• Percentage of activated cells (via phosphorylation)
• Percentage of non-expressing cells

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Muse™ PI3K Activation Dual Detection Kit

Assay Specific Module

• Bioprocess
• Research