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  • : ML-82K
  • : 250 tubes
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Mouse Leptin RIA


Description:
Mouse Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Mouse
Kit or Assay Type:
  • Endocrine Kits
  • Radioimmunoassays (RIA)
Therapeutic Areas:
  • Obesity
  • Metabolic Disorders
Linearity of Dilution:
100–125%
Label:
125I-Mouse Leptin
Sensitivity:
≤ 6.2 pM
Precision, %:
  • Inter-assay: 3.3–14.6
  • Intra-assay: 4.0–11.2
Accuracy:
92–94%
Detection Methods:
125I
Performance:
  • ED<sub>80</sub> = 1.2 ± 0.1 ng/ml
  • ED<sub>50 = 3.6 ± 0.2 ng/ml
  • ED <sub>20</sub> = 11.0 ± 0.6 ng/ml
Standard Curve Range:
0.2–20 ng/mL
Specificity:
Mouse Leptin 100% Rat Leptin 50% Human Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I, SRIF, Pancreatic Polypeptide Not Detectabl Somatostatin Not Detectabl Canine Leptin Not Detectable
Analytes Available:
Leptin