Ordering Information
Mouse Leptin RIA
Description:
Mouse Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Mouse
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
- Obesity
- Metabolic Disorders
Linearity of Dilution:
100–125%
Label:
125I-Mouse Leptin
Sensitivity:
≤ 6.2 pM
Precision, %:
- Inter-assay: 3.3–14.6
- Intra-assay: 4.0–11.2
Accuracy:
92–94%
Detection Methods:
125I
Performance:
- ED<sub>80</sub> = 1.2 ± 0.1 ng/ml
- ED<sub>50 = 3.6 ± 0.2 ng/ml
- ED <sub>20</sub> = 11.0 ± 0.6 ng/ml
Standard Curve Range:
0.2–20 ng/mL
Specificity:
Mouse Leptin 100% Rat Leptin 50% Human Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I, SRIF, Pancreatic Polypeptide Not Detectabl Somatostatin Not Detectabl Canine Leptin Not Detectable
Analytes Available:
Leptin