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Ordering Information
- : ML-82K
- : 250 tubes
Mouse Leptin RIA
Description:
Mouse Leptin RIA
Qty/Pk:
250 tubes
Background Information:
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Leptin (LEP; MIM 164160), an adipocyte-specific hormone that regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure, acts through the leptin receptor (LEPR), a single-transmembrane-domain receptor of the cytokine receptor family.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P48357 # Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).
SIZE: 1165 amino acids; 132494 Da
SUBUNIT: On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform E: Secreted (Probable).
TISSUE SPECIFICITY: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
DOMAIN: SwissProt: P48357 The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs. & The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell- surface receptor binding. & The box 1 motif is required for JAK interaction and/or activation.
PTM: On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
SIMILARITY: Belongs to the type I cytokine receptor family. Type 2 subfamily. & Contains 4 fibronectin type-III domains. & Contains 1 Ig-like (immunoglobulin-like) domain.
SIZE: 1165 amino acids; 132494 Da
SUBUNIT: On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform E: Secreted (Probable).
TISSUE SPECIFICITY: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
DOMAIN: SwissProt: P48357 The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs. & The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell- surface receptor binding. & The box 1 motif is required for JAK interaction and/or activation.
PTM: On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
SIMILARITY: Belongs to the type I cytokine receptor family. Type 2 subfamily. & Contains 4 fibronectin type-III domains. & Contains 1 Ig-like (immunoglobulin-like) domain.
Linearity of Dilution:
100–125%
Label:
125I-Mouse Leptin
Sensitivity:
≤ 6.2 pM
UniProt Number:
Precision, %:
- Inter-assay: 3.3–14.6
- Intra-assay: 4.0–11.2
Accuracy:
92–94%
Detection Methods:
- Radioactive
- 125I
Performance:
- ED80 = 1.2 ± 0.1 ng/ml
- ED50 = 3.6 ± 0.2 ng/ml
- ED20 = 11.0 ± 0.6 ng/ml
Product Name:
Mouse Leptin RIA
Standard Curve Range:
0.2–20 ng/mL
Specificity:
Mouse Leptin 100% Rat Leptin 50% Human Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I, SRIF, Pancreatic Polypeptide Not Detectabl Somatostatin Not Detectabl Canine Leptin Not Detectable
Analytes Available:
Leptin