Ordering Information
- : MPEQ-100K
- : 1 kit
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1 - Cell Signaling Multiplex Assay
| Number of Analytes Available | Research Areas | Detection Methods |
|---|---|---|
| 40 | Oncology | Fluorescent, Luminex xMAP |
Description:
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1 is a Luminex based approach for assessing the functional states of cells by profiling tyrosine phosphorylation changes across signaling pathways and cell phenotypes. Site-specific, quantitative measurements with picomolar sensitivity support diverse sample types and applications across the drug discovery / development continuum.
The MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1 is used for the analysis of cellular lysates prepared using the EpiQuant Sample Preparation Kit (MPEQ-SP, purchased separately). EpiQuant beads may be combined in desired configurations for the profiling of prepared lysate samples.
The MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1 is used for the analysis of cellular lysates prepared using the EpiQuant Sample Preparation Kit (MPEQ-SP, purchased separately). EpiQuant beads may be combined in desired configurations for the profiling of prepared lysate samples.
Background Information:
Multiplexing, using the Luminex® xMAP® platform, provides faster answers to cell signaling questions compared to traditional Western blots, mass spectrometry analyses and radioactive phosphorylation assays requiring large amounts of resources, time and sample.
However, multiplexing still presents some challenges. The requirement for native protein conformation can result in complex buffer interactions with phosphoproteins, as well as inter-antibody effects when multiplexed. Resulting difficulties include:
• Lack of high quality antibodies to a large number of targets with specific phosphorylation site modification for pathway analysis
• Qualitative, not quantitative results
• The need for separate wells to analyze each phosphorylated site on a specific analyte, as well as total protein
To overcome these multiplexing challenges, in addition to our MILLIPLEX® MAP cell signaling kits and MAPmates, Millipore proudly introduces our new MILLIPLEX® MAP EpiQuant™ Cell Signaling portfolio – a major advance in the design and implementation of quantitative immunoassays. The EpiQuant™ technology provides assays that are specific, quantitative and easily multiplexed.
MILLIPLEX® MAP EpiQuant™ assays allow customers to:
• Easily design panels with a high degree of “plex-ability” (e.g. > 4 times larger than traditional cell signaling multiplex assays).
• Customize their assay by selecting only the analytes they want
• Obtain quantitative results at picomolar sensitivities
• Measure phosphorylation site specific concentrations of proteins (even multiple phosphorylation sites on the same protein) — all in one well
• Measure both total and phosphoprotein — all in the same well (1H 2010)
However, multiplexing still presents some challenges. The requirement for native protein conformation can result in complex buffer interactions with phosphoproteins, as well as inter-antibody effects when multiplexed. Resulting difficulties include:
• Lack of high quality antibodies to a large number of targets with specific phosphorylation site modification for pathway analysis
• Qualitative, not quantitative results
• The need for separate wells to analyze each phosphorylated site on a specific analyte, as well as total protein
To overcome these multiplexing challenges, in addition to our MILLIPLEX® MAP cell signaling kits and MAPmates, Millipore proudly introduces our new MILLIPLEX® MAP EpiQuant™ Cell Signaling portfolio – a major advance in the design and implementation of quantitative immunoassays. The EpiQuant™ technology provides assays that are specific, quantitative and easily multiplexed.
MILLIPLEX® MAP EpiQuant™ assays allow customers to:
• Easily design panels with a high degree of “plex-ability” (e.g. > 4 times larger than traditional cell signaling multiplex assays).
• Customize their assay by selecting only the analytes they want
• Obtain quantitative results at picomolar sensitivities
• Measure phosphorylation site specific concentrations of proteins (even multiple phosphorylation sites on the same protein) — all in one well
• Measure both total and phosphoprotein — all in the same well (1H 2010)
Applications:
This MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1 is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: Cbl (Tyr700/731/774), Crk (Tyr221/239), CUB domain containing protein 1 (Tyr734), EGFR (Tyr1110/1125), Ezrin (Tyr477), FAK (Tyr397/407), FAK (Tyr861), FRS2 (Tyr436), Fyb (Tyr97/651), Gab1 (Tyr285/307/317), Gab2 (Tyr266), and more.
Key Applications:
Multiplexing
Application Notes:
• This kit may be used for the analysis of all or any combination of phosphotyrosine intracellular proteins in tissue/cell lysate and culture supernatant samples.
• This is a 3.5-hour assay.
• This assay requires 25 µl per well of lysed and digested sample.
• ONLY SAMPLES PREPARED USING THE EPIQUANT SAMPLE PREPARATION KIT (MPEQ-SP) WILL GENERATE RELIABLE RESULTS WITH THIS ASSAY.
• This is a 3.5-hour assay.
• This assay requires 25 µl per well of lysed and digested sample.
• ONLY SAMPLES PREPARED USING THE EPIQUANT SAMPLE PREPARATION KIT (MPEQ-SP) WILL GENERATE RELIABLE RESULTS WITH THIS ASSAY.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sensitivity:
Refer to kit protocol for sensitivities
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Modifications:
Phosphorylation
Cross Reactivity:
There is no significant cross-reactivity observed between the antibodies and/or non-specific analytes in this panel.
Product Name:
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Panel 1
Materials Required but Not Delivered:
MILLIPLEX® MAP EpiQuant™ Sample Preparation Kit (MPEQ-SP)
Analytes Available:
CUB domain containing protein 1 (Tyr734), Cbl (Tyr700/731/774), Crk (Tyr221/239), EGFR (Tyr1110/1125), Ezrin (Tyr477), FAK (Tyr397/407), FAK (Tyr861), FRS2 (Tyr436), Fyb (Tyr97/651), Gab1 (Tyr285/307/317), Gab2 (Tyr266), Gab2 (Tyr584), Growth hormone receptor (Tyr566), HER2 (Tyr1023), HGFR (Tyr1003), IL-15Rα chain (Tyr227), IRS1 (Tyr612), ITK (Tyr180), JAK3 (Tyr785), JNK 1/2/3 (Tyr185/185/223), LAT (Tyr255), PDK1 (Tyr373/376), PI3K p110γ (Tyr485), PLCγ1 (Tyr1253), PLCγ2 (Tyr1197/1217), Plakoglobin/γ-catenin (Tyr548), Raf-1 (Tyr340/341), Rap GEF1/GRF2 (Tyr504), SCF38 (Tyr20), SH2 domain containing protein 3A (Tyr231), SH2 domain containing protein 3A (Tyr95), SPTAN1 (Tyr1176), STAT3 (Tyr705), Shc (Tyr349/350), Transferrin Receptor (Tyr20), Tyrosine protein kinase Tec (Tyr206), VEGFR1 (Tyr1169), WASP (Tyr291), ZAP70 (Tyr126), p38/MAPKα (Tyr181)
Configuration:
Design your multiplex kit by choosing from 40 analytes.
Components:
- Cell Signaling Assay Buffer 1 (43-010)
- EpiQuant Dilution Buffer (MPEQ-DB)
- EpiQuant Phosphotyrosine Detection Antibody (25X) (MPEQ-1100)
- Streptavidin-Phycoerythrin (SAPE) (45-001D)
- EpiQuant Phosphotyrosine Panel 1 Standard (MPEQ-8100)
- EpiQuant Phosphotyrosine Panel 1 Quality Controls 1 and 2 (MPEQ-6100)
- Set of one 96-Well filter Plate with 2 sealers (MX-PLATE)
UniProt Number:
Panel Type:
Cell Signaling
Precision, %:
- Intra-Assay 1.8% - 17.5%
- Inter-Assay 5.6% - 19.6%
Accuracy:
81.2% - 19.6%
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
96 well plate
Entrez Gene Number:
- NM_005188
- NM_005206
- NM_178181
- NM_005228
- NM_003379
- NM_153831
- NM_006654
- NM_002037
- NM_207123
- NM_080491
- NM_000163
- NM_004448
- NM_001127500
- NM_002189
- NM_005544
- NM_005546
- NM_000215
- NM_139049
- NM_014387
- NM_001315
- NM_002613
- NM_005026
- NG_009090
- NM_002660
- NM_002661
- NM_002880
- NM_005312
- NM_018976
- NM_005490
- NM_183001
- NM_001130438
- NM_007315
- NM_001128148
- NM_003215
- NM_002019
- NM_000377
- NM_001079
- NM_139068
- NM_002753
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