Description:
Mouse Soluble Cytokine Receptor Premixed 13 Plex Kit
Trade Name:
MILLIPLEX
Background Information:
Cytokines play a fundamental role in the pathogenesis of many diseases and are key targets for drug development. The discovery that soluble cytokine receptors are involved in regulating excessive inflammatory responses and modulating immune events has stimulated significant research interest in their potential role as immunotherapeutic agents. Many of these soluble cytokine receptors have the ability to inhibit the binding and biological activity of their cytokine ligands, making them very specific cytokine antagonists. The Mouse Soluble Cytokine Receptor Premixed 13 Plex Kit quantitatively measures the soluble cytokine receptor biomarkers in mouse serum, plasma, cell/tissue culture supernatant, tissue/cell lysate, or other body fluid samples, in a premixed kit.
Key Applications:
Multiplexing
Application Notes:
25 µL sample volume 1:5 serum or plasma in assay buffer; 25 µL cell/tissue culture supernatant or lysate
Species Reactivity:
Mouse
Precision, %:
-
Intra-assay: 5 - 9
-
Inter-assay: 6 - 10
Sensitivity:
-
sCD30 - 9 pg/ml
-
sgp130 - 84 pg/ml
-
sIL-1RI - 3 pg/ml
-
sIL-1RII - 11 pg/ml
-
sIL-2Rα - 6 pg/ml
-
sIL-4R - 3 pg/ml
-
sIL-6R - 24 pg/ml
-
sRAGE - 24 pg/ml
-
sTNFRI - 22 pg/ml
-
sTNFRII - 5 pg/ml
-
sVEGFR1 - 76 pg/ml
-
sVEGFR2 - 33 pg/ml
-
sVEGFR3 - 42 pg/ml
Therapeutic Areas:
Inflammation
Accuracy:
-
sCD30 - 94%
-
sgp130 - 82%
-
sIL-1Rl - 93%
-
sIL-1Rll - 100%
-
sIL-2Rα - 95%
-
sIL-4R - 100%
-
sIL-6R - 105%
-
sRAGE - 78%
-
sTNFRI - 103%
-
sTNFRII - 96%
-
sVEGFR1- 176%
-
sVEGFR2 - 103%
-
sVEGFR3 - 101%
Standard Curve Range:
-
4.9 - 20,000 pg/mL - sTNFRI
-
12.2 - 50,000 pg/ml - sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sTNFRII, sCD30, sgp130, sIL-6R, sRAGE, sVEGFR1
-
24.4 - 100,000 pg/ml - sVEGFR2, sVEGFR3
Kit Type:
-
Chemokine Kits
-
Cytokine Kits
Assay Panel:
Premixed beads for your convenience.
Array Type:
Antibody Arrays
Configuration:
Pre-mixed 13 Plex
Analytes Available:
-
sCD30 (sTNFRSF8)
-
sgp130
-
sIL-1RI (sCD121a)
-
sIL-1RII (sCD121b)
-
sIL-2Rα (sCD25)
-
sIL-4R (sCD124)
-
sIL-6R (sCD126)
-
sRAGE
-
sTNFRI (sTNFRSF1A)
-
sTNFRII (sTNFRSF1B)
-
sVEGFR1 (sFlt-1)
-
sVEGFR2 (sFlk-1)
-
sVEGFR3 (sFlt-4)
Detection Methods:
Luminex xMAP
Linearity of Dilution:
73–126%
Cross Reactivity:
Non-detectable or negligible cross-reactivity within the panel
Protein:
Milliplex Map Kits
Assay Type:
Multiplex Assays
