Porcine Insulin RIA

Porcine Insulin RIA

Insulin is a 5.8 kD hormone is secreted by the islet cells in the pancreas.

Radioimmunoassay

Overnight, equilibrium incubation at 4°C. Sample volume < 100 µl serum, plasma, or tissue culture media.

• Pig
• Pig

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

• Diabetes
• Obesity
• Metabolic Disorders

2–200 µU/mL

¹²⁵I-Insulin

Porcine Insulin 100% Procine Proinsulin 53% Human Insulin 100% Bovine Insulin 90% Bovine Proinsulin 50% Intact Human Proinsulin 38% Rat Insulin 19% Des 31, 32 HPI 47% Des 64, 65 HPI 72% Glucagon Not Detectable Pancreatic Polypeptide Not Detectable Somatos

Endocrine Kits

Insulin

¹²⁵I

• Endocrine Kits
• Radioimmunoassays (RIA)

• ED₈₀ = 7 ± 0.6 µU/ml
• ED₅₀ = 30 ± 1.2 µU/ml
• ED₂₀ = 126 ± 3.0 µU/ml

Radioimmunoassays (RIA)