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  • : PI-12K
  • : 250 tubes

Porcine Insulin RIA

Description:
Porcine Insulin RIA
Qty/Pk:
250 tubes
Background Information:
Insulin is a 5.8 kD hormone is secreted by the islet cells in the pancreas.
Applications:
This radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight, equilibrium incubation at 4°C. Sample volume < 100 μl serum, plasma, or tissue culture media.
Species Reactivity:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Label:
125I-Insulin
Standard Curve Range:
2–200 µU/mL
Product Name:
Porcine Insulin RIA
Specificity:
Porcine Insulin 100% Procine Proinsulin 53% Human Insulin 100% Bovine Insulin 90% Bovine Proinsulin 50% Intact Human Proinsulin 38% Rat Insulin 19% Des 31, 32 HPI 47% Des 64, 65 HPI 72% Glucagon Not Detectable Pancreatic Polypeptide Not Detectable Somatos
Analytes Available:
Insulin
UniProt Number:
Detection Methods:
  • Radioactive
  • 125I
Performance:
  • ED80 = 7 ± 0.6 µU/ml
  • ED50 = 30 ± 1.2 µU/ml
  • ED20 = 126 ± 3.0 µU/ml

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