Primate Leptin RIA
Description:
Primate Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight, equilibrium incubation at 4°C. Sample volume < 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Primate
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Label:
125I-Human Leptin
Specificity:
Primate Leptin 100% Human Leptin 100% Mouse Leptin <0.2% Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-1 Not DetectableSomatostatin, PP Not DetectableCanine Leptin Not Detectable
Kit Type:
Endocrine Kits
Detection Methods:
125I
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Performance:
- ED<sub>80</sub> = 1.1 ± 0.1 ng/ml
- ED<sub>50 = 4.7 ± 0.3 ng/ml
- ED <sub>20</sub> = 20.8 ± 1.7 ng/ml
Assay Type:
Radioimmunoassays (RIA)