EmbryoMax® Primary Mouse Embryo Fibroblasts, Not Mytomycin C Treated, Strain CF1, passage 1
Description:
EmbryoMax® Primary Mouse Embryo Fibroblasts, Not Mytomycin C Treated, Strain CF1, passage 1
Qty/Pk:
1 vial
Product Overview:
PMEF-CFL-P1 are low passage Primary Mouse Embryo Fibroblasts (PMEFs) isolated from CF1 mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
Applications:
The PMEF-CFL-P1 fibroblasts are low passage Primary Mouse Embryo Fibroblasts (PMEFs). These fibroblasts are isolated from CF1 mouse embryos at day 13.
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Brand Family:
Specialty Media
Presentation:
Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Storage Conditions:
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging:
1x106 cells
Product Name:
EmbryoMax® Primary Mouse Embryo Fibroblasts, Not Mytomycin C Treated, Strain CF1, passage 1
Alternate Names:
PMEF's Neo Resistant - Not Mitomycin C treated