Rat Insulin RIA
Description:
Rat Insulin RIA
Background Information:
Insulin, a 5.8 kD hormone is secreted by the islet beta cells in the pancreas.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight, equilibrium incubation at 4º C. Sample volume 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Rat
Precision, %:
- Inter-assay: 8.5–9.4
- Intra-assay: 1.4–4.6
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Accuracy:
85–93%
Standard Curve Range:
0.1–10 ng/mL
Label:
125I-Insulin
Specificity:
Rat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Human Proinsulin 69% Procine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectabl Glucagon Not Detectable Somatostatin Not Detectable Pancr
Kit Type:
Endocrine Kits
Detection Methods:
125I
Linearity of Dilution:
80 -100%
Performance:
ED80 = 0.2 ± 0.02 ng/mL
ED50 = 0.8 ± 0.04 ng/mL
ED20 = 3.0 ± 0.36 ng/mL
ED50 = 0.8 ± 0.04 ng/mL
ED20 = 3.0 ± 0.36 ng/mL
Assay Type:
Radioimmunoassays (RIA)