Bestellinformationen
Rat/Mouse Obestatin RIA
Description:
Rat/Mouse Obestatin RIA
Background Information:
Obestatin is a relatively newly discovered peptide whose biological functions are not fully understood. Rat obestatin, a 23-amino acid peptide produced in the stomach, is coded by the same ghrelin gene and also derived from pre-pro-ghrelin. After differential modification, obestatin and ghrelin activate different receptors, resulting in opposite effects on appetite induction. Whereas ghrelin has an appetite-inducing effect, obestatin has been hypothesized to have an anorectic effect characterized by suppressed food intake, slower gastric emptying, inhibited jejunal motility and decreased body-weight gain. Consequently, obestatin and the ghrelin-to-obestatin ratio may play a significant role in the etiology and pathogenesis of obesity.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium assay incubate at 4°C.
Species Reactivity:
- Rat
- Mouse
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
- Obesity
- Gasterointestinal Disorders
Linearity of Dilution:
Please contact Technical Service for this information
Label:
125I-Human Obestatin
Sensitivity:
0.078 ng/mL with 50 µL sample volume
Precision, %:
- Intra-assay: <9
- Inter-assay: <8
Accuracy:
74-97%
Detection Methods:
125I
Performance:
- ED80 = 2.61 ± 0.17 ng/mL
- ED50= 0.62 ± 0.02 ng/mL
- ED20= 0.16 ± 0.01 ng/mL
Standard Curve Range:
0.078 - 10.0 ng/mL