Información pedidos
OxyBlot Protein Oxidation Detection Kit
Description:
OxyBlot Protein Oxidation Detection Kit
Trade Name:
- OxyBlot
- Chemicon (Millipore)
Product Overview:
The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
Key Applications:
Western Blotting
Application Notes:
Preparation of protein lysate:
The protein lysate may be prepared using a variety of protocols, such as those detailed in ref. 38 and 39. Most standard buffers such as RIPA and Triton are also suitable. It is recommended that a reducing agent be added to the lysis buffer to prevent the oxidation of proteins that may occur after cell lysis. Lysis buffer containing either 1-2% 2-mercaptoethanol or 50 mM DTT sufficiently inhibits this oxidation, but has no adverse effect on the derivatization reaction in the OxyBlot™ protocol. Purified protein samples are also suitable for analysis with the OxyBlot™ Kit.
The protein lysate may be prepared using a variety of protocols, such as those detailed in ref. 38 and 39. Most standard buffers such as RIPA and Triton are also suitable. It is recommended that a reducing agent be added to the lysis buffer to prevent the oxidation of proteins that may occur after cell lysis. Lysis buffer containing either 1-2% 2-mercaptoethanol or 50 mM DTT sufficiently inhibits this oxidation, but has no adverse effect on the derivatization reaction in the OxyBlot™ protocol. Purified protein samples are also suitable for analysis with the OxyBlot™ Kit.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
- Oxidative Stress Kits
- Apoptosis Assays
- Neurobiology Kits
- Apoptosis Assays
Storage Conditions:
Store all components at 2°C to 8°C.
Detection Methods:
Chemiluminescent