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Ordering Information

  • : SCC064
  • : ≥1X106 cells/vial

Product Images

Typical morphology of LX-2 cells 72 hours after thawing.

LX-2 cells express high levels of α-SMA (1:50, MAB1501X).

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LX-2 Human Hepatic Stellate Cell Line

Description:
LX-2 Human Hepatic Stellate Cell Line
Qty/Pk:
≥1X106 cells/vial
Background Information:
This product is available for sale to academic institutions or not-for-profit entities for research use. For information on commercial licensing please contact Marie Azzaria (Marie.Azzaria@emdmillipore.com) or Jamie Hoberg (James.Hoberg@emdmillipore.com).

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
Usage Statement:
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.
Components:
1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.
Storage Conditions:
LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.
Product Name:
LX-2 Human Hepatic Stellate Cell Line
Alternate Names:
  • HSC
  • Perisinusoidal cells
  • Ito cells
  • Hepatic lipocytes
  • Hepatic pericytes