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Arctic Ground Squirrel Neural Stem Cells (AGS-NSC)
Description:
Arctic Ground Squirrel Neural Stem Cells (AGS-NSC)
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 vial containing 500,000 cells
Product Overview:
Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells (AGS-NSC) are isolated from the hippocampus of adult Arctic Ground Squirrels following hibernation. Both the isolated cells and the whole animals are tolerant of ischemic insult and reperfusion. Using these cells, researchers can identify potentially novel proteins and genes key to neuronal tolerance, neuroprotection and neurogenesis. Millipore’s AGS-NSC can be used to screen drugs, genes and proteins for protective changes caused by oxygen and/or glucose deprivation. These cells can potentially identify future targets for novel stroke therapeutics. As stem cells, these cells can be used in transplantation studies using rodent stroke models to investigate tolerance to ischemic injury.
This product is for Research Use Only. This product is not approved for human or veterinary use, or for use in in vitro diagnostic or clinical procedures.
This product is for Research Use Only. This product is not approved for human or veterinary use, or for use in in vitro diagnostic or clinical procedures.
Key Applications:
Stem Cell Culture
Application Notes:
Please see data sheet for detailed directions on Thawing, Plating , Feeding, Passaging, etc.
AGS-NSC should be cultured in AGS-NSC Expansion medium (warmed to 37°C) for best results. Please see specific media data sheets for further information (SCMA002 and SCMA003).
AGS-NSC should be cultured in AGS-NSC Expansion medium (warmed to 37°C) for best results. Please see specific media data sheets for further information (SCMA002 and SCMA003).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Arctic Ground Squirrel
Quality Assurance:
AGS-NSC are tested for normal NSC morphology, and tested negative for mycoplasma, bacterial and fungal growth. They maintain normal morphology in expansion medium (SCMA002) and differentiate into neurons in AGS-NSC differentiation medium (SCMA003).
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Cells/Isolation
Cell Line Type:
Neural Stem Cells
Components:
SCCE002
Brand Family:
Chemicon
Source:
Hippocampus of Arctic Ground Squirrels
Storage Conditions:
AGS-NSC’s are sold as cryopreserved ampoules. Ampoules are shipped in insulated packages containing dry ice to insure the cells remain in a cryopreserved state. To maintain the integrity of the cells, unpack the products immediately upon receipt. The cryopreserved cells should be placed in the liquid or gas phase of a liquid nitrogen dewar for storage. If the cells are to be thawed and plated within 24 hours, they may be stored at -80°C.
Do not store the ampoule for more than 24 hours at -80°C as the cells will slowly degrade at this temperature. Long-term storage of the ampoule must be in a liquid nitrogen dewar or a mechanical freezer designed for cryopreserved cell storage that maintains a temperature below -135°C.
Millipore recommends storing the cryopreserved vials in liquid nitrogen vapor phase. Handle cryopreserved vials with caution. Always wear eye protection and gloves when working with cell cultures. Aseptically vent any nitrogen from cryopreserved vials in a biosafety cabinet prior to thawing the vials in a water bath. If vials must be stored in liquid phase, the vials should be transferred to vapor phase storage or -80°C for at least 24 hours prior to being thawed.
Do not store the ampoule for more than 24 hours at -80°C as the cells will slowly degrade at this temperature. Long-term storage of the ampoule must be in a liquid nitrogen dewar or a mechanical freezer designed for cryopreserved cell storage that maintains a temperature below -135°C.
Millipore recommends storing the cryopreserved vials in liquid nitrogen vapor phase. Handle cryopreserved vials with caution. Always wear eye protection and gloves when working with cell cultures. Aseptically vent any nitrogen from cryopreserved vials in a biosafety cabinet prior to thawing the vials in a water bath. If vials must be stored in liquid phase, the vials should be transferred to vapor phase storage or -80°C for at least 24 hours prior to being thawed.
Packaging:
500,000 cells
Product Name:
Arctic Ground Squirrel Neural Stem Cells (AGS-NSC)
Materials Required but Not Delivered:
Arctic Ground Squirrel Neural Stem Cell Expansion Medium (CHEMICON Cat. No. SCMA002)
Arctic Ground Squirrel Neural Stem Cell Differentiation Medium (CHEMICON Cat. No. SCMA003)
Arctic Ground Squirrel Neural Stem Cell Differentiation Medium (CHEMICON Cat. No. SCMA003)