HEScGRO Medium for Human ES Cell Culture
Description:
HEScGRO Medium for Human ES Cell Culture
Product Overview:
HEScGRO hES cell medium is the first, animal-component free medium that is specially formulated to meet the unique requirements of human embryonic stem cell culture. No other hES cell media is available as a serum-free, ready-to-use, and complete formulation. HEScGRO has been extensively tested and proven to maintain the pluripotential nature of several hES cell lines including: MEL-1, MEL-2 and H1. This medium is fully-defined, serum-free, and animal-component free, and does not require additional supplementation to maintain cells in their pluripotent state. Mitotically inactive feeder cell layers are commonly used to support hES cell growth. PLEASE NOTE: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.
Key Applications:
Stem Cell Culture
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
Cell Culture Media
Media Form:
Liquid
Packaging:
5 x 100 mL Aliquots
Materials Required but Not Delivered:
Tissue Culture plates
Matrigel, 0.1 percent gelatin solution, human collagen IV or other tissue culture plastic coating material.
Mitocally inactivated Detroit 551 feeder cells are recommended (ATCC cat no. CCL-11) plated at 80,000 cells/cm2
Other feeder cells such as HS27 or WS1 may also be used, typically plated at 55,000 cell/cm2 IMPORTANT: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.
Matrigel, 0.1 percent gelatin solution, human collagen IV or other tissue culture plastic coating material.
Mitocally inactivated Detroit 551 feeder cells are recommended (ATCC cat no. CCL-11) plated at 80,000 cells/cm2
Other feeder cells such as HS27 or WS1 may also be used, typically plated at 55,000 cell/cm2 IMPORTANT: Human feeder cells are required for successful hES cell culture. Mouse feeder cells are not recommended with this medium.
Media Type:
Stem Cell Culture Media
Formulation:
HEScGRO Medium is a proprietary, patent-pending formulation. The media formulation does not contain any animal components. It comes as a complete, ready-to-use formulation with (20 ng/mL) bFGF included.
Quality Assurance:
Each lot of medium is tested for the optimal cell growth and proliferation of MEL-1 Human ES cells
Sterility Testing: Negative
Osmolarity: 260-270 mOsm
pH: 7.2-7.3
Sterility Testing: Negative
Osmolarity: 260-270 mOsm
pH: 7.2-7.3
Storage Conditions:
HEScGRO Serum-Free Medium should be stored at -20°C until ready to use. Upon thawing, the medium should be stored at 2-8°C and given a 2 week expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.