Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium

Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium

500 mL

Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium is a formulation that is designed for the spontaneous or directed differentiation of human embryonic stem cells

Embryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation embryos and have the ability to differentiate into cells comprising all three embryonic germ layers. One method to identify the pluripotent potential of hES cells is the induction of in vitro differentiation through the culture of human ES cells in suspension to form embryoid bodies. Embryoid bodies are subsequently evaluated for the presence of markers for ectoderm, mesoderm, and endoderm. Additionally, embryoid bodies are considered to provide an environment that mimics early embryonic development and can hence be used for the differentiation of hES cells towards specific lineages.

Stem Cell Culture

Millipore’s Human ES Cell Embryoid Body Formation Medium has been optimized and qualified to support the formation of embryoid bodies. The medium can be used to form embryoid bodies in suspension culture on low adhesion plates. Embryoid bodies formed using SCM026 have been shown to facilitate the differentiation of human ES cells into neural, endodermal, and cardiac cell lineages.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Each lot of medium is tested for the ability to support human ES cell embryoid body formation. Sterility Testing: Negative.

hES Cell Culture Media

• Human Embryonic Stem Cells
• Induced Pluripotent Stem Cells

• Maintenance & Expansion
• Differentiation

Stem Cell Culture Media

Liquid

Human Embryonic Stem Cell Embryoid Body Formation Medium should be stored at -20°C until ready to use. Upon thawing, the medium should be stored at 2-8°C and given a 3 week expiration dating. Dispense into aliquots to avoid repeated heating prior to each use. The media can also be dispensed into aliquots and refrozen at -20°C although repeat freeze-thaws are not recommended.

Five 100mL bottles

Cell Culture Media

Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium

Human ES Cell Embryoid Body Formation Medium is a proprietary formulation that is based on published human ES cell embryoid body media (1-4) and contains 20% fetal bovine serum (ES Qualified), glutamine, nonessential amino acids, and β-mercaptoethanol.

• Cryopreserved Human Embryonic Stem Cells (Catalog Nos. SCC020 and SCC021).
• HEScGRO MediumTM for Human ES Cell Culture (Catalog No. SCM020).
• Accumax™ (Catalog No. SCR006).
• Dulbecco’s Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B).
• Tissue culture plates.
• Matrigel, 0.1 % gelatin solution, human collagen IV, or other tissue culture plastic coating material.
• Mitotically inactivated Detroit 551 feeder cells are recommended (ATCC cat no. CCL-110) plated at 60,000 cells/cm² .
• Low attachment plates.