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Collagenase type I
Description:
Collagenase type I
Trade Name:
Chemicon (Millipore)
Qty/Pk:
250 mg
Product Overview:
Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
Key Applications:
Stem Cell Culture
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore's HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
Cell Culture Media or Reagent Type:
hES Cell Culture Media
Stem Cell Type:
- Human Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Stem Cell Culture Media
Brand Family:
Chemicon
Storage Conditions:
Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.
Packaging:
1 vial containing 250mg of lyophilized powder
Cell Culture Reagent Type:
Cell Culture Media
Product Name:
Collagenase type I
Formulation:
Collagenase type I is supplied as a dialyzed, lyophilized powder.
Materials Required but Not Delivered:
• Ultrapure water (e.g., MilliQ)
• 0.22 μM sterile filtration device (e.g. Millipore’s Stericup-GP, Catalog # SCGPU01RE)
• 0.22 μM sterile filtration device (e.g. Millipore’s Stericup-GP, Catalog # SCGPU01RE)