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  • : SCR600
  • : 1 kit
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Human Oligodendrocyte Differentiation Kit

Description:
Human Oligodendrocyte Differentiation Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
EMD Millipore’s Human Oligodendrocyte Differentiation Kit contains Human Oligodendrocyte Progenitor Cells (OPCs) along with optimized media for their expansion and spontaneous differentiation into mature oligodendrocytes. Human Oligodendrocyte Progenitor Cells (OPCs) are derived from NIH approved H9 human embryonic stem cells (hESCs). These hESC-derived OPCs proliferate as an adherent cell monolayer and greater than 70% express the appropriate early-to-intermediate oligodendrocyte markers, including O4, NG2, Olig2, Sox10 and GalC. Upon growth factor withdrawal, Human OPCs spontaneously differentiate into mature oligodendrocytes expressing terminally differentiated markers including proteolipid protein (PLP), myelin basic protein (MBP), and myelin oligodendrocyte glycoprotein (MOG). Spontaneous differentiation leads to approximately 30% of the cells expressing PLP, MBP and MOG while the remaining 70% express mostly neuronal markers, MAP2 and beta III-tubulin with very little to no astrocytes (<5% GFAP-positive cells) observed. Human OPCs can be used for a variety of research applications including studies of neurotoxicity, co-culture applications and screening for molecules that induce or inhibit preferential differentiation to mature oligodendrocytes.
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Sterility testing: Negative for bacteria and fungal growth
pH: 7.8 +/- 0.3
Osmolality: 270 +/- 10mOsm
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Differentiation
Cell Line Type:
Neural Stem Cells
Components:
  • Human Oligodendrocyte Progenitors (OPCs), 500,000 viable cells: (Cat. No. CS204496)
  • Human OPC Expansion Media Kit, 100 mLs: (Cat. No. SCM107)
  • Human OPC Spontaneous Differentiation Media Kit, 100 mLs: (Cat. No. SCM106)
Brand Family:
Chemicon
Storage Conditions:
Please see manual for recommended storage conditions for individual components
Cell Culture Reagent Type:
Stem Cell Reagents
Product Name:
Human Oligodendrocyte Differentiation Kit