Ordering Information
- : SF001-500P
- : 500 mL
ESGRO Complete PLUS Clonal Grade Medium
Recommended Replacement for: SF001-500Description:
ESGRO Complete PLUS Clonal Grade Medium
Trade Name:
Chemicon (Millipore)
Qty/Pk:
500 mL
Product Overview:
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency.
Key Applications:
Stem Cell Culture
Application Notes:
Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth.
Media can be used on feeder dependent/independent ES cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Media can be used on feeder dependent/independent ES cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
QC PROTOCOL:
Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.
Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.
Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.
Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.
Cell Culture Media or Reagent Type:
Mouse ES Cell Culture Media
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Cell Culture Media
Components:
- 1. ESGRO Complete Clonal Grade Medium: (Catalogue No. SF001-B) One (1) 500 mL bottle containing original clonal grade medium. Complete formulation containing self-renewal factors, no need to add LIF. pH 7.1-7.3
- 2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-250) One (1) 250 µL vial containing selective GSK3β inhibitor.
Brand Family:
Chemicon
Storage Conditions:
• Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels.
• Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
• Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
• After supplement is added to clonal grade medium, store in the dark at 4°C for up to
two weeks. Do not refreeze.
• Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
• Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
• After supplement is added to clonal grade medium, store in the dark at 4°C for up to
two weeks. Do not refreeze.
Cell Culture Reagent Type:
Cell Culture Media
Replaces:
SF001-500
Product Name:
ESGRO Complete PLUS Clonal Grade Medium