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Ordering Information
- : SF006
- : 100 mL
ESGRO Complete Accutase
Description:
ESGRO Complete Accutase
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 mL
Product Overview:
ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
Key Applications:
Stem Cell Culture
Application Notes:
Cell Detachment:
1. Thaw Accutase™ to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
1. Thaw Accutase™ to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Media or Reagent Type:
Cell Dissociation Reagents
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Brand Family:
Chemicon
Presentation:
Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
Storage Conditions:
Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase™ may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.
Cell Culture Reagent Type:
Cell Culture Dissociation Reagents
Product Name:
ESGRO Complete Accutase