Sensitive Human Leptin RIA
Description:
Sensitive Human Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Multiplexing
Application Notes:
Two-day disequilibrium incubation at room temperature. Sample volume 100 µL serum, plasma, or tissue culture media
Species Reactivity:
Human
Precision, %:
- Inter-assay: 3.2–8.9
- Intra-assay: 3.7–7.5
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Accuracy:
104–118%
Standard Curve Range:
0.05–10 ng/mL
Label:
125I-Human Leptin
Specificity:
Human Leptin 100% Mouse Leptin <2% Human Insulin Not Detectable Glucagon Not Detectable Rat Leptin <1% SRIF Not Detectable Canine Leptin <0.2%
Kit Type:
Endocrine Kits
Gene Symbol:
- LEP
- leptin
- OB
- obesity
- OBS
Detection Methods:
125I
Linearity of Dilution:
72–104%
Performance:
- ED80 = 0.28 ± 0.04 ng/ml
- ED50 = 1.05 ± 0.12 ng/ml
- ED20 = 4.48 ± 0.66 ng/ml
Assay Type:
Radioimmunoassays (RIA)