Sensitive Rat Insulin RIA

Sensitive Rat Insulin RIA

Insulin, a 5.8 kDa hormone is secreted by the islet beta cells in the pancreas. This kit offers sensitive assay for rat/mouse insulin

Radioimmunoassay

Two-day disequilibrium incubation at 4°C. Sample volume 100 µL serum, plasma, or tissue culture media.

Human

• Inter-assay: 3.8–10.8
• Intra-assay: 2.7–5.8

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

• Diabetes
• Obesity
• Metabolic Disorders

88–108%

0.02–1.0 ng/mL

¹²⁵I-Insulin

Rat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Porcine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectable Glucagon Not Detectable Somatostatin Not Detectable Pancreatic Polypeptide No

Endocrine Kits

¹²⁵I

84–119%

• Endocrine Kits
• Radioimmunoassays (RIA)

• ED₈₀ = 0.09 ± 0.01 ng/mL
• ED₅₀ = 0.22 ± 0.01 ng/mL
• ED₂₀ = 0.55 ± 0.01 ng/mL

Radioimmunoassays (RIA)