Multi-Species Leptin RIA
Description:
Multi-Species Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubationat 4°C. Sample volume 100 µL serum, plasma, or tissue culture media
Species Reactivity:
- Human
- Mouse
- Rat
- Pig
Precision, %:
- Inter-assay: 6.5–8.7
- Intra-assay: 2.8–3.6
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Accuracy:
83–104%
Standard Curve Range:
1–50 ng/mL (Human equivalent)
Label:
125I-Mouse Leptin
Specificity:
Human Leptin 100% Porcine Leptin 67% Rat Leptin 61% Mouse Leptin 73% Human Insulin Not Detectable Human Proinsulin Not Detectable Human C-Peptide Not Detectable Rat Insulin Not Detectable Glucagon Not Detectable IGF-I Not Detectable Canine Lepti
Kit Type:
Endocrine Kits
Detection Methods:
125I
Linearity of Dilution:
94–115%
Performance:
- ED80 = 3.0 ± 0.28 ng/mL HE
- ED50 = 8.1 ± 0.60 ng/mL HE
- ED20 = 24.4 ± 2.0 ng/mL HE, HE = Human Equivalent
Assay Type:
Radioimmunoassays (RIA)