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Flow Cytometry: Flow cytometry analysis using anti-phosphoserine, clone 4A4. Cells were either untreated (shaded, green) or treated (unshaded) with Ca...
Immunohistochemistry: Untreated and phosphorylated serine pre-treated (panels A and B, respectively) 4A4 staining pattern/morphology on paraffin embe...
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Anti-Phosphoserine, clone 4A4
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | WB, IF, FC, IH(P), ELISA | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphoserine, clone 4A4
Background Information:
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
Clone:
4A4
Specificity:
Serine-phosphorylated proteins from all species
Key Applications:
- Western Blotting
- Immunofluorescence
- Flow Cytometry
- Immunohistochemistry (Paraffin)
- ELISA
Species Reactivity:
Vertebrates
Isotype:
IgG1
Immunogen:
Phosphoserine coupled to KLH
Modifications:
Phosphorylation
Molecular Weight:
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Control:
Suggested Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is recommended as a positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
Presentation:
1 mL of PBS
Storage Conditions:
2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Quality Assurance:
Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Antibody Category:
Signaling
Antibody Sub-Category:
- General Post-translation Modification
- Kinases & Phosphatases
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Mouse
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein G-Sepharose™ chromatography