Bestellinformationen
Produktabbildungen
WB: Jurkat cell lysate probed with anti-Lck at 0.5μg/ml
Anti-Lck, clone 3A5
| Speziesreaktivität | Hauptanwendungen | Wirt | Format | Antikörperart |
|---|---|---|---|---|
| H, M, R | IP, WB | Mouse | Purified | Monoclonal Antibody |
UniProt-Nummer:
UniProt Summary
FUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and clas
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GenBank-Nummer:
Entrez Gene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds
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Description:
Anti-Lck, clone 3A5
Clone:
3A5
Specificity:
Recognizes Lck.
Key Applications:
- Immunoprecipitation
- Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG2bκ
Immunogen:
GST-fusion to residues 1-225 of murine Lck
Molecular Weight:
56 kDa
Control:
Positive Control Included: Jurkat cell lysate (12-303)
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- LCK
- pp58lck
- LSK
- p56-LCK
- p56lck
- YT16
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Antibody Category:
Signaling
Antibody Sub-Category:
Immunological Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified