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Product Images
WB: A431 cell lysate was probed with anti-Caspase 9 (2μg/ml). Arrows indicate the proform and cleaved Caspase 9 (~46kDa and 34kDa).
Anti-Caspase 9, clone 96-2-22
| Reatividade à Espécie | Aplicações Essenciais | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| H | WB | Mouse | Purified | Monoclonal Antibody |
Número Uniprot:
UniProt Summary
FUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
SIZE: 416 amino acids; 46281 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 an ... see more »
SIZE: 416 amino acids; 46281 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 an ... see more »
Número Genbank:
Entrez Gene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alte
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Description:
Anti-Caspase 9, clone 96-2-22
Clone:
96-2-22
Specificity:
Recognizes the proform and the active cleaved form of Caspase 9.
Key Applications:
Western Blotting
Species Reactivity:
Human
Isotype:
IgG
Immunogen:
The N-terminal fragment of human Caspase 9 (amino acid residues 1-134)
Molecular Weight:
46/34 kDa
Control:
Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
Presentation:
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- CASP9
- CASP-9
- ICE-LAP6
- MCH6
- APAF3
- CASPASE-9c
- OTTHUMP00000044594
- APAF-3
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from A431, Raji, or HFF cells
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- Caspases
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G Purified