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Imágenes del producto
WB: C4 cell lysate was probed with Anti-IGF-1 R, clone JBW902. Arrow indicates beta chain IGF-1 receptor (95 kDa).
Anti-IGF-IR, clone JBW902
| Reactividad de especies | Aplicaciones claves | Huésped | Formato | Tipo de anticuerpo |
|---|---|---|---|---|
| H, M | IH, IP, WB | Mouse | Purified | Monoclonal Antibody |
Número UniProt:
UniProt Summary
FUNCTION: SwissProt: P08069 # This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.
SIZE: 1367 amino acids; 154793 Da
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the bet ... ver más »
SIZE: 1367 amino acids; 154793 Da
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the bet ... ver más »
Número GenBank:
Entrez Gene Summary
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Description:
Anti-IGF-IR, clone JBW902
Background Information:
There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Clone:
JBW902
Specificity:
Recognizes IGF-1 receptor b chain; does not cross react with insulin receptor.
Key Applications:
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Species Reactivity:
- Human
- Mouse
Isotype:
IgG2a
Immunogen:
GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor
Molecular Weight:
95 kDa
Control:
C4 cell lysate, HepG2 cells
Presentation:
Protein A Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.14 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- IGF1R
- IGFIR
- MGC18216
- MGC142170
- JTK13
- MGC142172
- CD221
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF-1 Receptor)
Antibody Category:
Signaling
Antibody Sub-Category:
Insulin/Energy Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified