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Flow Cytometry: Jurkat cells were cultured for 18 hr without (panel A) or with (panel B) Fas Ligand vesicles (1:500 dilution; Cat. # 01-210), then sur...
Anti-Phosphatidylserine, clone 1H6, was used at two concentrations (as indicated) to quantitate apoptosis induced in Jurkat cells by either Fas Ligand...
Anti-Phosphatidylserine, clone 1H6
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| Vrt | FC, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphatidylserine, clone 1H6
Background Information:
Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.
Clone:
clone 1H6
Specificity:
Recognizes phosphatidylserine (PS) in cell membranes
Key Applications:
- Flow Cytometry
- Immunohistochemistry
Application Notes:
Flow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Species Reactivity:
Vertebrates
Isotype:
IgG
Immunogen:
Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol
Modifications:
Phosphorylation
Molecular Weight:
none given
Control:
Brain tissue
Presentation:
Purified mouse monoclonal IgG in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
Storage Conditions:
Stable for 1 year at from date of receipt.
Quality Assurance:
routinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
Apoptosis - Additional
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Product Overview:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Purification Method:
Protein G Purified