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WB: SFME cell lysate probed with anti-Cystatin C at 2μg/ml
Anti-Cystatin C
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, R, M | ELISA, IH, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P01034 # As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
SIZE: 146 amino acids; 15799 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Found in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular g ... もっと見る »
SIZE: 146 amino acids; 15799 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Found in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular g ... もっと見る »
Entrez Gene Number:
Entrez Gene Summary
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they
...
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Description:
Anti-Cystatin C
Background Information:
Cystatin C (CST3 or Gamma trace) is a serum protein used mainly as a measure of glomerular filtration rate. Studies have shown that Cystatin C allows a more precise testing of kidney function than creatinine. Cystatin C is a single 120-residue polypeptide belonging to the type 2 cystatin gene family. Type 2 cystatin proteins are a class of cysteine proteinase inhibitors that provide protective functions and are found in a variety of human fluids and secretions. A mutation in the gene associated with type 2 cystatin has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in patients with vascular disease. Mutations in the cystatin 3 gene are responsible for the Icelandic type of hereditary cerebral amyloid angiopathy, a condition that can lead to susceptibility to cerebral hemorrhage.
Alternate Names:
- Neuroendocrine basic polypeptide
- bA218C14.4 (cystatin C)
- cystatin 3
- cystatin C
- cystatin C (amyloid angiopathy and cerebral hemorrhage)
Specificity:
Recognizes Cystatin C, Mr of 14 kDa and its precursor.
Key Applications:
- ELISA
- Immunohistochemistry
- Western Blotting
Application Notes:
Immunohistochemistry:
10 µg/mL of a previous lot detected Cystatin C in paraformaldehyde-fixed rat brain section.
ELISA:
Recommended.
10 µg/mL of a previous lot detected Cystatin C in paraformaldehyde-fixed rat brain section.
ELISA:
Recommended.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, Mouse, Rat. Reactivity with other species has not been confirmed.
Isotype:
IgG
Immunogen:
Human Cystatin C isolated from the urine of a patient with tubular proteinuria.
Molecular Weight:
14 kDa
Control:
SFME cell lysates
Presentation:
Purified rabbit polyclonal IgG in buffer containing DEAE depleted antibodies reactive with human plasma proteins in PBS, pH 7.4, 0.05% sodium azide. Frozen solution.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- CST3
- Post-gamma-globulin
- gamma-trace
- MGC117328
- Gamma-trace
- HCCAA
- post-gamma-globulin
- Cystatin-3
Quality Assurance:
Routinely evaluated by western blot on serum-treated mouse SFME cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Cystatin C in mouse brain extract. A previous lot detected Cystatin C in mouse spleen and thymus extracts.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Cystatin C in mouse brain extract. A previous lot detected Cystatin C in mouse spleen and thymus extracts.
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- BCL2 & Inhibition
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
DEAE chromatography