Informazioni per l’ordine
Immagini del prodotto
WB: NIH 3T3 cell lysate probed with anti-FAK (1μg/ml)
Anti-FAK
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| H, M, R, Ht, non Av | WB, IP, IC | Rabbit | Purified | Polyclonal Antibody |
Numero UniProt:
UniProt Summary
FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in incr
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Numero GenBank:
Entrez Gene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain
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Description:
Anti-FAK
Background Information:
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it's phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
Alternate Names:
- FADK 1
- PTK2 protein tyrosine kinase 2
- Protein-tyrosine kinase 2
- focal adhesion kinase 1
Specificity:
Recognizes and is specific for human p125FAK, does not cross-react with Pyk2.
Key Applications:
- Western Blotting
- Immunoprecipitation
- Immunocytochemistry
Application Notes:
Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
Species Reactivity:
- Human
- Mouse
- Rat
- Hamster
Species Reactivity Note:
Human, mouse, rat and hamster not avian.
Non-Reactive Species:
Avian
Isotype:
IgG
Immunogen:
pGEX-derived fusion protein containing residues 748-1053 of human FAK
Molecular Weight:
125 kDa
Control:
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation:
Purified rabbit polyclonal IgG in buffer containing storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Store at -20°C.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- PTK2
- pp125FAK
- FADK
- FAK1
- FAK
Quality Assurance:
Evaluated by western blot on RIPA lysates from murine 3T3/A31 or human A431 RIPA cell lysate.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.
Antibody Category:
Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A purified