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Western Blot Analysis Untreated (lane 1), and staurosporine-treated (lane 2) Jurkat cell lysates were probed with Anti-phospho H2AX (Ser139).
Western Blot Analysis:
Untreated and staurosporine treated Jurkat lysate(Lane 1 and 2) was resolved by electrophoresis, transferred to PVDF membra...
Anti-phospho-H2A.X (Ser139)
Recommended Replacement for: 07-164 is a recommended replacement for AB3369| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt, H | IP, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucl
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Entrez Gene Number:
Entrez Gene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A fam
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Description:
Anti-phospho-H2A.X (Ser139)
Trade Name:
Upstate (Millipore)
Background Information:
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
Key Applications:
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Species Reactivity:
- Vertebrates
- Human
Species Reactivity Note:
Human; broad species cross-reactivity expected based on conservation of sequence homology.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Molecular Weight:
15 kDa
Control:
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Isotype:
IgG
Modifications:
Phosphorylation
Replaces:
07-164 is a recommended replacement for AB3369
Gene Symbol:
- HIST2H2AC
- H2A-GL101
- H2AFQ
- H2A/r
- H2A/q
- MGC74460
- H2A
Format Code:
Pur
Alternate Names:
- H2A histone family, member Q
- histone 2, H2ac
- histone IIa
- histone cluster 2, H2ac
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Specificity:
Histone H2A.X phosphorylated at Ser139.
Quality Assurance:
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Immunogen:
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Format:
Purified
Host:
Rabbit
Epitope:
a.a. 134-142
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Histones
Purification Method:
Protein A chromatography