Ordering Information
Goat Anti-Mouse IgG, HRP conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, IH, WB | Goat | HRP | Polyclonal Antibody |
Description:
Goat Anti-Mouse IgG, HRP conjugate
Background Information:
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity:
Recognizes mouse IgG, both heavy and light chains.
Key Applications:
- ELISA
- Immunohistochemistry
- Western Blotting
Application Notes:
Additional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
Species Reactivity:
Mouse
Species Reactivity Note:
Mouse
Immunogen:
Highly purified whole mouse IgG.
Presentation:
Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Quality Assurance:
Western Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Antibody Category:
Secondary & Control Antibodies
Antibody Sub-Category:
Whole Immunoglobulin Secondary Antibodies
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format:
HRP
Host:
Goat
Antibody Type:
Polyclonal Antibody