Guide d'achat
Calyculin A/Okadaic acid-treated A431 Cell Lysate
Description:
Calyculin A/Okadaic acid-treated A431Cell Lysate
Trade Name:
Upstate (Millipore)
Key Applications:
Western Blotting
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lysate Type:
Cell Lysate
Species:
Human
Quality Assurance:
Routinely evaluated as a positive control for some of Upstates antibodies.
Cell Type:
Human carcinoma cell line
Presentation:
100 μg in 100 μL of lysate diluted to 1mg/mL in reducing sample buffer. Concentration: 1 mg/mL. Frozen solution.
Storage Conditions:
6 months at -20°C from date of shipment.
Long Description:
Cellular protein preparation. Cells were serum starved overnight with serum-free media. Cells were cultured for 2 hours in the presence of a final concentration of 10nM Calyculin A and 500nM Okadaic acid. Cells were washed with ice-cold PBS and lysed in RIPA buffer. Protein concentration was determined and diluted with 2X SDS-PAGE sample loading buffer with 5% 2-Mercaptoethanol. Lysate was then boiled for 5 minutes.
Concentration:
1 mg/mL