Bestellinformationen
PI3 Kinase (p110α (E545K)/p85α) Murine
Description:
PI3 Kinase (p110α(E545K)/p85α), Murine
Product Overview:
Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α containing the mutation E545K and untagged, recombinant, full length mouse p85α.
Background Information:
The E545K substitution is associated with a number of human cancers. This residue is conserved between the human and mouse sequences of p110α. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Key Applications:
Kinase Assay
Species:
Mouse
Molecular Weight:
p110α (Ε545Κ) MW = 129kDa,
p85α MW = 83.6kDa.
p85α MW = 83.6kDa.
Therapeutic Areas:
Inflammation
Source:
Coexpressed by baculovirus in Sf21 insect cells.
Storage Conditions:
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging:
Also available in 250 μg size --call for pricing and availability and reference catalog number 14-781M when ordering the 250 μg size.
Gene Symbol:
- PIK3CA
- PI3K
- MGC142161
- p110-alpha
- MGC142163
Protein or Enzyme Type:
Kinases
Purification Method:
Ni2+ /NTA agarose
Protein Type:
Kinase