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Bestellnummer : 16-218
St./Pkg. : 100 µg
Listenpreis : RMB 4,548.96
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05-806	Anti-phospho-Histone H3 (Ser10), clone 3H10	100 µg
16-222	Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate	100 μg

Produktabbildungen

IB: Acid extracts from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and...

Anti-Phospho-Histone H3 (Ser10), clone 3H10, Cy5 conjugate

Speziesreaktivität	Hauptanwendungen	Wirt	Format	Antikörperart
H	IF, WB	Mouse	Purified	Monoclonal Antibody

UniProt-Nummer:

Q16695

UniProt Summary

FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family. ... weitere anzeigen »

GenBank-Nummer:

NM_003493.2

Entrez Gene Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member ... weitere anzeigen »

Description:

Anti-Phospho-Histone H3 (Ser10), clone 3H10, Cy5 conjugate

Background Information:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity:

Histone H3 phosphorylated at Ser10

Key Applications:

Immunofluorescence
Western Blotting

Species Reactivity:

Human

Species Reactivity Note:

Expected to cross react with all vertebrate species.

Isotype:

IgG1κ

Immunogen:

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806.

Modifications:

Phosphorylation

Molecular Weight:

17kDa

Control:

Acid extracts from colcemid treated HeLa cells (lane 2)

Storage Conditions:

1 year at 4°C from date of shipment

Gene Symbol:

HIST3H3
H3FT
H3/t
H3t
MGC126888
H3/g
H3.4
H3T
MGC126886

Quality Assurance:

Routinely evaluated by immunoblot in acid extrated protein from mitotic HeLa cells treated with colcemid (17-306).

Antibody Category:

Epigenetics & Nuclear Function

Antibody Sub-Category:

Histones

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Upstate (Millipore)

Antibody Type:

Monoclonal Antibody

Format:

Purified

Host:

Mouse

Purification Method:

protein G purified

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Antikörper(52)

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Bestellinformationen

Produktabbildungen

Anti-Phospho-Histone H3 (Ser10), clone 3H10, Cy5 conjugate

Produktinformationsquellen

Analysenzertifikate

Häufig gestellte Fragen/FAQ

Genverwandte Produkte für dieses Target anzeigen

Gene Related Products

Kompatible Sekundärantikörper

Sekundärantikörper suchen

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