Informazioni per l’ordine
Immagini del prodotto
IF: Mitotic HeLa cells stained with 4μg/ml of this lot (green) and DAPI (blue).
WB: Acid extract from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells were probed with anti-phospho-Histone H3, clone 3H10, FITC Conjugate...
Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| H | WB, IC, IF | Mouse | Purified | Monoclonal Antibody |
Numero UniProt:
UniProt Summary
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 ami ... visualizza altro »
SIZE: 136 ami ... visualizza altro »
Numero GenBank:
Entrez Gene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member
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Description:
Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Alternate Names:
- H3 histone family, member T
- histone 3, H3
- histone cluster 3, H3
Clone:
clone 3H10
Specificity:
Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunofluorescence
Application Notes:
Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.
Species Reactivity:
Human
Species Reactivity Note:
Human. Broad species cross-reactivity is expected.
Isotype:
IgG
Immunogen:
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.
Epitope:
Ser10
Modifications:
Phosphorylation
Molecular Weight:
17 kDa
Control:
Mitotic HeLa cells (IF).
Presentation:
Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- HIST3H3
- H3FT
- H3/t
- H3t
- MGC126888
- H3/g
- H3.4
- H3T
- MGC126886
Quality Assurance:
Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Histones
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G Purified