ChIPAb+ Estrogen Receptor α

P03372

FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
SIZE: 595 amino acids; 66216 Da
SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. ... hide » see more »

NM_000125.2

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. ... hide » see more »

ChIPAb+ Estrogen Receptor α

Estrogen Receptor α

• Chromatin Immunoprecipitation (ChIP)
• Western Blotting

• Human
• Mouse

The ERα antibody is made against aa120-170 of bovine estrogen α.

66kDa

• 1 vial containing isotype control monoclonal antibody, ascites, 100 μL. Store at -20°C.
• 1 vial containing 75 μL of 5μM of each control primer specific for human pS2 promoter. Store at -20°C.
• FOR: CCG GCC ATC TCT CAC TAT GAA
• REV: CCT TCC CGC CAG GGT AAA TAC

1 vial containing 100 μL ascites.

1 year at -20°C from date of shipment

• ESR1
• Era
• ER-alpha
• ESR
• ER
• NR3A1
• ESRA
• DKFZp686N23123

Routinely evaluated by chromatin immunoprecipitation and immunoblot on MCF7 cell lysate.

• Anti-estrogen receptor α monoclonal antibody, 1 vial
• Isotype negative control monoclonal antibody, 1 vial
• pS2 promoter primer set, 1 vial

Epigenetics & Nuclear Function

Chromatin Biology

Upstate (Millipore)

Ascites

Mouse

Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. ERα antibody is functionally validated in the precipitation of ERα associated chromatin.
The qPCR primers included flank the ERα binding site in human pS2 promoter.

25 assays per kit. ~4μl per chromatin immunoprecipitation.

Monoclonal Antibody