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Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 μg/ml). Proteins were visualized using ...
ChIPAb+ LEF1
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| H, M | WB, Chromatin Immunoprecipitation (ChIP) | Mouse | Purified | Monoclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform
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Code GenBank:
Entrez Gene Summary
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
Description:
ChIPAb+ LEF1
Specificity:
Recognizes LEF1 (lymphoid enhancer-binding factor 1).
Key Applications:
- Western Blotting
- Chromatin Immunoprecipitation (ChIP)
Species Reactivity:
- Human
- Mouse
Isotype:
IgG1
Immunogen:
The antibody is made against amino acid residues 1-85 of human LEF1.
Molecular Weight:
55-57kDa
Control:
- One vial containing 125μg of normal mouse IgG in 125μl volume. Store at -20°C.
- 1 vial containing 75μl of 5μM of each control primer specific for human c-myc promoter. Store at -20°C.
- FOR: CCC AAA AAA AGG CAC GGA A
- REV: TAT TGG AAA TGC GGT CAT GC
Presentation:
phosphate buffered saline with sodium azide.
Storage Conditions:
1 year at -20°C from date of shipment
Gene Symbol:
- LEF1
- DKFZp586H0919
- TCF1ALPHA
- LEF-1
- TCF1-alpha
Quality Assurance:
Routinely evaluated by chromatin immunoprecipitation on HT29 cells and immunoblot on Jurkat cell lysate.
Components:
- Anti-LEF1 monoclonal antibody, 1 vial
- Isotype control monoclonal antibody, 1 vial
- Human c-myc promoter primer set, 1 vial
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
- Chromatin Biology
- Transcription Factors
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Mouse
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.
Packaging:
25 assays per kit. ~4μg per chromatin immunoprecipitation.
Antibody Type:
Monoclonal Antibody