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Imágenes del producto
Acid-extracted histones from Hela cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti trimethyl-Histone H3 (Lys...
Chromatin Immunoprecipitation:
Sonicated Chromatin prepared from 3x106 NIH3T3 L1 cells were subjected to chromatin immunoprecipitation using 4 µg ...
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ChIPAb+ Trimethyl-Histone H3 (Lys9)
| Reactividad de especies | Aplicaciones claves | Huésped | Formato | Tipo de anticuerpo |
|---|---|---|---|---|
| Vrt | Chromatin Immunoprecipitation (ChIP), Mplex, WB | Rabbit | Purified | Polyclonal Antibody |
Número UniProt:
UniProt Summary
FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 ami ... ver más »
SIZE: 136 ami ... ver más »
Número GenBank:
Entrez Gene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyad
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Description:
ChIPAb+ Trimethyl-Histone H3 (Lys9)
Background Information:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Trimethyl-Histone H3 (Lys9) set includes the Anti-trimethyl-Histone H3 (Lys9) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 170 bp region within the promoter of the murine p16 gene. The trimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys9) associated chromatin.
The ChIPAb+ Trimethyl-Histone H3 (Lys9) set includes the Anti-trimethyl-Histone H3 (Lys9) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 170 bp region within the promoter of the murine p16 gene. The trimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys9) associated chromatin.
Specificity:
Recognizes histone H3, Mr 17 kDa, trimethylated at lysine 9.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Multiplexing
- Western Blotting
Species Reactivity:
Vertebrates
Species Reactivity Note:
Human, mouse. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Isotype:
IgG
Immunogen:
The trimethyl-histone H3 (Lys9) purified antibody is made against BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl lysine 9 of human Histone H3.
Epitope:
Trimethyl Lys9
Molecular Weight:
17kDa
Control:
Included negative control rabbit IgG antibody and control primers specific for murine p16 promoter.
Presentation:
Anti-trimethyl-Histone H3 (Lys9) (rabbit polyclonal IgG), Cat.# CS200604. One vial containing 100 μg protein A purified IgG in 100 μL of 0.02 M Phosphate buffer, pH7.4, 0.25 M NaCl, 0.05% sodium azide with 30% glycerol. Store at -20°C
.
Normal Rabbit IgG, Cat. #PP64B. One vial containing 125 μg of normal rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, p16 (mouse) Cat. # CS200602. One vial containing 75 μL of 5 μM of each primer specific for mouse p16 promoter. Store at -20°C.
FOR: ACA CTC CTT GCC TAC CTG AA
REV: CGA ACT CGA GGA GAG CCA TC
.
Normal Rabbit IgG, Cat. #PP64B. One vial containing 125 μg of normal rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, p16 (mouse) Cat. # CS200602. One vial containing 75 μL of 5 μM of each primer specific for mouse p16 promoter. Store at -20°C.
FOR: ACA CTC CTT GCC TAC CTG AA
REV: CGA ACT CGA GGA GAG CCA TC
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Aliquot upon thawing, avoid freeze thaw cycles.
Gene Symbol:
- H3F3A
- MGC87783
- H3.3A
- MGC87782
- H3F3
- H3.3B
- H3F3B
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated Chromatin prepared from 3x106 NIH3T3 L1 cells were subjected to chromatin immunoprecipitation using 4 µg of either normal rabbit IgG, (Cat. #PP64B), or Anti-trimethyl-Histone H3 (Lys9) antibody (Cat. #CS200604) and the Magna ChIP A kit (Cat. #17-610). Successful enrichment of trimethyl-histone H3 (Lys9)-associated DNA fragments was verified by qPCR using ChIP Primers mouse p16 (Cat.# CS200602) flanking the mouse p16 promoter (Figure 1).
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Sonicated Chromatin prepared from 3x106 NIH3T3 L1 cells were subjected to chromatin immunoprecipitation using 4 µg of either normal rabbit IgG, (Cat. #PP64B), or Anti-trimethyl-Histone H3 (Lys9) antibody (Cat. #CS200604) and the Magna ChIP A kit (Cat. #17-610). Successful enrichment of trimethyl-histone H3 (Lys9)-associated DNA fragments was verified by qPCR using ChIP Primers mouse p16 (Cat.# CS200602) flanking the mouse p16 promoter (Figure 1).
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Components:
- Anti-trimethyl Histone H3 (Lys9) polyclonal, 1 vial
- Murine p16 promoter primer set, 1 vial
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Histones
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Trimethyl-Histone H3 (Lys9) antibody is functionally validated in the precipitation of chromatin that carries trimethyl-Histone H3 (Lys9).
The qPCR primers included amplifies the mouse p16 promoter region where trimethyl-Histone H3 (Lys9) is enriched.
The qPCR primers included amplifies the mouse p16 promoter region where trimethyl-Histone H3 (Lys9) is enriched.
Packaging:
25 assays per kit, ~4μl per chromatin immunoprecipitation.