Ordering Information
- : ESG1106
- : 106 units
- : 1 million units/1 mL
Product Images
Undifferentiated ES cells cultured for five days in media containing CHEMICON's ESGRO® (ESG1106 & ESG1107). A concentration of 1000 Units/mL is used f...
ES (R1) cell colonies (x10) cultured on gelatinized plates using ESGRO® (1000 units/mL).
ESGRO® (LIF)
Description:
ESGRO® (LIF)
Trade Name:
Chemicon (Millipore)
Key Applications:
Stem Cell Culture
Application Notes:
Applications for ESGRO® include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).
At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.
Visit www.esgro-lif.com for additional information
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).
At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.
Visit www.esgro-lif.com for additional information
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Mouse
Cell Culture Media or Reagent Type:
ESGRO Supplement
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Cell Culture Media Additives
Format:
Purified
Presentation:
ESGRO® is supplied in liquid form as 106 Units ESGRO® in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Storage Conditions:
ESGRO® is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO concentrate be stored at 4°C. Freeze-thawing will reduce potency.It is recommended that prior to use, ESGRO™ should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided.ESGRO™ is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
WARRANTY
The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.NOTE: ESGRO® should not be allowed to come in contact with ruminant animals or swine.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale. Use of recombinant LIF, or CHEMICON's proprietary ESGRO® formulation, for the maintenance of embryonic stem cells is protected world-wide under the following patents: US 5,166,065, EP380646, and others.
WARRANTY
The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.NOTE: ESGRO® should not be allowed to come in contact with ruminant animals or swine.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale. Use of recombinant LIF, or CHEMICON's proprietary ESGRO® formulation, for the maintenance of embryonic stem cells is protected world-wide under the following patents: US 5,166,065, EP380646, and others.
Specific Activity:
ESGRO® is assessed both on ES {embryonic stem} cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Packaging:
1 mL
Cell Culture Reagent Type:
- Cytokines & Growth Factors
- Cell Culture Supplements
Gene Symbol:
- LIF
- Emfilermin
- CDF
- MLPLI
- HILDA
- D-FACTOR
Protein or Enzyme Type:
Cytokines & Growth Factors
Alternate Names:
Murine LIF
Concentration:
1 million units/1 mL
Purity:
The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative for mycoplasmas.