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Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with anti-AIF (Catalog Number AB16501) at 1 μg...
Rabbit anti-AIF (Catalog Number AB16501) staining of human breast carcinoma. Tissue pretreated with citrate buffer, ph 6.0, anti-AIF at 20μg/ml, IHC ...
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Anti-AIF, internal domain
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH(P), WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: O95831 # Probable oxidoreductase that acts as a caspase- independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro).
COFACTOR: FAD.
SIZE: 613 amino acids; 66901 Da
SUBUNIT: Binds to DNA in a sequence-independent manner.
SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note=Translocated to the nucleus ... see more »
COFACTOR: FAD.
SIZE: 613 amino acids; 66901 Da
SUBUNIT: Binds to DNA in a sequence-independent manner.
SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note=Translocated to the nucleus ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for
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Description:
Anti-AIF, internal domain
Background Information:
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999).
Alternate Names:
Apoptosis Inducing Factor
Specificity:
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al. 1999).
Key Applications:
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Western blot: 0.25 to 1 μg/mL.
K562 cell lysate can be used as a positive control and a 67 kDa band should be detected.
Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs.
Optimal working dilutions must be determined by end user.
K562 cell lysate can be used as a positive control and a 67 kDa band should be detected.
Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
Immunogen:
Rabbit anti-AIF (Internal region) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (Susin et al. 1999). This sequence is identical to those of mouse and rat AIF (Susin et al. 1999).
Epitope:
internal domain
Molecular Weight:
67 kDa
Control:
Widely expressed
Presentation:
Purified IgG in PBS containing 0.02% sodium azide.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- AIFM1
- MGC111425
- PDCD8
- AIF
- EC 1.-.-.-
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
Apoptosis - Additional
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Ammonium sulfate precipitation and DEAE-cellulose chromatography