Informazioni per l’ordine
Immagini del prodotto
Western blot analysis of Bim in K562 (K) and A549 (A) whole cell lysates with anti-Bim (IN) at 1:1000 dilution.
Anti-Bim, internal epitope, pan-Bim isoforms
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Numero UniProt:
UniProt Summary
FUNCTION: SwissProt: P09211 # Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
SIZE: 210 amino acids; 23356 Da
SUBUNIT: Homodimer.
SIMILARITY: SwissProt: P09211 ## Belongs to the GST superfamily. Pi family.
SIZE: 210 amino acids; 23356 Da
SUBUNIT: Homodimer.
SIMILARITY: SwissProt: P09211 ## Belongs to the GST superfamily. Pi family.
Numero GenBank:
Entrez Gene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are th
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Description:
Anti-Bim, internal epitope, pan-Bim isoforms
Alternate Names:
BOD
Specificity:
Recognizes pan-Bim isoforms.
Key Applications:
Western Blotting
Application Notes:
Western blot: 1:1000-1:2000
Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.
Optimal working dilutions must be determined by end user.
Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
Immunogen:
Synthetic peptide corresponding to amino acids 22-40 of human origin (O'Conneor et al. 1998). The sequence is identical to that of mouse and is different by one amino acid from that of rat.
Epitope:
internal epitope, pan-Bim isoforms
Molecular Weight:
23 kDa
Control:
Skin/Skin Tumor
Presentation:
Immunoaffinity Purified immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
The undiluted antibody solution is stable for approximately 6 months when stored 2–8°C.
Gene Symbol:
- GSTP1
- DFN7
- GST3
- GSTP1-1
- FAEES3
- PI
- EC 2.5.1.18
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
BCL2 & Inhibition
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Rabbit
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Purification Method:
Immunoaffinity Purified