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In cultures of breast cancer MB-MDA-468 cells treated with protein kinase inhibitor staurosporine, only cells with chromatin condensation typical of a...
FIGURE 1. Fluorescence microphotographs of MB-MDA-468 cells treated with staurosporine, heated in formamide, stained with MAB3299 (clone F7-26) and co...
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Anti-DNA, single stranded specific, clone F7-26
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| A | FC, IC, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-DNA, single stranded specific, clone F7-26
Background Information:
Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.
Clone:
F7-26
Specificity:
Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
1. Immunoprecipitation
2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
Species Reactivity:
All
Isotype:
IgM
Immunogen:
F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.
Epitope:
single stranded specific
Control:
Apoptotic cells
Presentation:
Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.
Storage Conditions:
Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
Apoptosis - Additional
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Format Code:
Pur
Concentration:
100 μg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Ammonium sulfate precipitation